Daphnia magna, a freshwater crustacean, has been widely used as invertebrate model in aquatic toxicology and environmental monitoring. Its small size, simple anatomical organization, yet high sensitivity to toxicity makes it a perfect research model, but it is lacking a reference atlas. To fill this gap, we are creating whole-organism atlas utilizing 2D and 3D imaging. This atlas at cellular resolution will serve as a foundation for the characterization of tissue and cellular change in Daphnia.
Micro-Computed Tomography (micro-CT) is rapidly becoming commonplace in biological applications where the generation of high resolution, isotropic, 3D datasets is useful for both qualitative and quantitative phenotyping as well as visualization. While there is a fairly wide selection of commercial micro-CT scanners, optimization of sample preparation and scanning parameters can require a larger and more specialized setup than what is readily available. The focus of the Micro-CT Project is to construct and continuously develop a system that optimizes high (cellular) resolution while maintaining a large field of few. The resulting images will allow us to determine both the composition and health of individuals in samples using phenotyping tools.
Whole organism atlas utilizing 2D and 3D imaging. Atlas at cellular resolution serves as foundation for characterization of tissue and cellular change in Daphnia.
| Resource Name: | Daphnia Atlas |
|---|---|
| Version: | 1.0.0 |
| Research Resource Identifier (RRID): | RRID:SCR_024913 |
| Dataset citation: | A web-based histology atlas for the freshwater Cladocera species Daphnia magna, version 1.0.0 |
| Paper citation | A web-based histology atlas for the freshwater Cladocera species Daphnia magna Mee S. Ngu, Daniel J. Vanselow, Carolyn R. Zaino, Alex Y. Lin, Jean E. Copper, Margaret J. Beaton, Luisa Orsini, John K. Colbourne, Keith C. Cheng, Khai C. Ang bioRxiv 2022.03.09.483544v4; DOI:10.1101/2022.03.09.483544 |
These materials are provided as-is, without direct support.
Project Coordinator
Mee S. Ngu, PhD
Developer
Daniel J. Vanselow
Annotation
Mee S. Ngu, PhD
Carolyn R. Zaino
Principal Investigators
Khai C. Ang, PhD
Keith C. Cheng, MD PhD
Slide Scanning
Jean Copper
Histology Processing
Mee S. Ngu, PhD
Debra A. Shearer
Chadwick Harris
Contact Us
Mee S. Ngu: msn21@psu.edu
Khai C. Ang: kca2@psu.edu
Jake Gittlen Laboratories for Cancer Research
Penn State Human Health and Environment Seed Grant supported by Pennsylvania Department of Health Commonwealth Universal Research Enhancement Program Grant.
The Department of Health specifically disclaims responsibility for any analyses, interpretations, or conclusions.